Coding

Part:BBa_K2929001

Designed by: James Hammond   Group: iGEM19_St_Andrews   (2019-10-10)


Mak33 IgG CH3 Domain

CH3 Domain of the Mouse (Mus musculus) anticreatine kinase (Mak33) IgG antibody. PDB ID: 1CQK. Codons optimised for Escherichia coli.

5' NcoI and 3' BamHI cut site.


Usage and Biology

The Mak33 IgG CH3 Domain expresses in well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.

Figure 1. Mouse IgG wild-type expression duplicates showing insoluble recombinant protein at 15 kDa and no soluble expression based on the soluble fraction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 66
    Illegal BamHI site found at 322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//proteindomain
Parameters
protein